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Swelling-activated and arachidonic acid-induced currents are TREK-1 in rat bladder smooth muscle cells
https://fmu.repo.nii.ac.jp/records/2001910
https://fmu.repo.nii.ac.jp/records/20019107467a299-3a66-4da2-83a7-6157303f6c04
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FksmJMedSci_62_p18.pdf (823.8 KB)
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| Item type | デフォルトアイテムタイプ（フル）fmu(1) | |||||||||||
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| 公開日 | 2016-07-06 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Swelling-activated and arachidonic acid-induced currents are TREK-1 in rat bladder smooth muscle cells | |||||||||||
| 言語 | en | |||||||||||
| 作成者 |
Fukasaku, Mitsuko
× Fukasaku, Mitsuko
× Kimura, Junko
× Yamaguchi, Osamu
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| 権利情報 | ||||||||||||
| 権利情報 | © 2016 The Fukushima Society of Medical Science | |||||||||||
| 内容記述 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Using the perforated patch voltage clamp, we investigated swelling-activated ionic channels (SACs) in rat urinary bladder smooth muscle cells. Hypo-osmotic (60%) bath solution increased a membrane current which was inhibited by the SAC inhibitor, gadolinium. The reversal potential of the hypotonicity-induced current shifted in the positive direction by increasing external K+ concentration. The hypotonicity-induced current was inhibited by extracellular acidic pH, phorbol ester and forskolin. These pharmacological properties are identical to those of arachidonic acid-induced current present in these cells, suggesting the presence of TREK-1, a four-transmembrane two pore domain K+ channel. Using RT-PCR we screened rat bladder smooth muscles and cerebellum for expression of TREK-1, TREK-2 and TRAAK mRNAs. Only TREK-1 mRNA was expressed in the bladder, while all three were expressed in the cerebellum. We conclude that a mechanosensitive K+ channel is present in rat bladder myocytes, which is activated by arachidonic acid and most likely is TREK-1. This K+ channel may have an important role in the regulation of bladder smooth muscle tone during urine storage. | |||||||||||
| 出版者 | ||||||||||||
| 出版者 | The Fukushima Society of Medical Science | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 書誌情報 |
en : Fukushima Journal of Medical Science 巻 62, 号 1, p. 18-26, 発行日 2016 |
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| 関連情報 | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.5387/fms.2015-20 | |||||||||||
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| 識別子タイプ | PMID | |||||||||||
| 関連識別子 | 26911303 | |||||||||||
| 関連情報 | ||||||||||||
| 識別子タイプ | ICHUSHI | |||||||||||
| 関連識別子 | 2017326461 | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 出版タイプ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 0016-2590 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||
| 収録物識別子 | 2185-4610 | |||||||||||
| 収録物識別子 | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AA0065246X | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | K+ channel | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | TREK-1 | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | rat urinary bladder | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | smooth muscle | |||||||||||
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| 主題Scheme | Other | |||||||||||
| 主題 | perforated patch clamp | |||||||||||
| 主題 | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | arachidonic acid | |||||||||||
