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Effects of a high-sodium diet on renal tubule Ca2+ transporter and claudin expression in Wistar-Kyoto rats
https://fmu.repo.nii.ac.jp/records/2000019
https://fmu.repo.nii.ac.jp/records/20000191f489529-659a-47c5-a6f3-4e83f365d044
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1471-2369-13-160.pdf (944.0 KB)
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| Item type | デフォルトアイテムタイプ（フル）fmu(1) | |||||||||||||||||||||||
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| 公開日 | 2013-07-01 | |||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||
| タイトル | Effects of a high-sodium diet on renal tubule Ca2+ transporter and claudin expression in Wistar-Kyoto rats | |||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||
| 作成者 |
Yatabe, Midori Sasaki
× Yatabe, Midori Sasaki
× Yatabe, Junichi
× Takano, Kozue
× Murakami, Yuta
× Sakuta, Rina
× Abe, Sadahiko
× Sanada, Hironobu
× Kimura, Junko
× Watanabe, Tsuyoshi
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| 権利情報 | ||||||||||||||||||||||||
| 権利情報Resource | http://creativecommons.org/licenses/by/2.0 | |||||||||||||||||||||||
| 権利情報 | © 2012 Yatabe et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||||||||||||||||||||
| 内容記述 | ||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||
| 内容記述 | Background: Urinary Ca^{2+} excretion increases with dietary NaCl. NaCl-induced calciuria may be associated with hypertension, urinary stone formation and osteoporosis, but its mechanism and long-term effects are not fully understood. This study examined alterations in the expressions of renal Ca^{2+} transporters, channels and claudins upon salt loading to better understand the mechanism of salt-induced urinary Ca^{2+} loss. Methods: Eight-week old Wistar-Kyoto rats were fed either 0.3% or 8% NaCl diet for 8 weeks. Renal cortical expressions of Na+/Ca2+ exchanger 1 (NCX1), Ca^{2+} pump (PCMA1b), Ca^{2+} channel (TRPV5), calbindin-D28k, and claudins (CLDN-2, -7, -8, -16 and −19) were analyzed by quantitative PCR, western blot and/or immunohistochemistry. Results: Fractional excretion of Ca^{2+} increased 6.0 fold with high-salt diet. Renal cortical claudin-2 protein decreased by approximately 20% with decreased immunological staining on tissue sections. Claudin-16 and −19 expressions were not altered. Renal cortical TRPV5, calbindin-D28k and NCX1 expressions increased 1.6, 1.5 and 1.2 fold, respectively. Conclusions: Chronic high-salt diet decreased claudin-2 protein and increased renal TRPV5, calbindin-D28k, and NCX1. Salt loading is known to reduce the proximal tubular reabsorption of both Na+ and Ca^{2+}. The reduction in claudin-2 protein expression may be partly responsible for the reduced Ca^{2+} reabsorption in this segment. The concerted upregulation of more distal Ca^{2+}-transporting molecules may be a physiological response to curtail the loss of Ca^{2+}, although the magnitude of compensation does not seem adequate to bring the urinary Ca^{2+} excretion down to that of the normal-diet group. | |||||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||||
| 出版者 | BioMed Central Ltd. | |||||||||||||||||||||||
| 言語 | ||||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||||
| 書誌情報 |
en : BMC nephrology 巻 13, p. 160, 発行日 2012-12-02 |
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| 関連情報 | ||||||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||||
| 関連識別子 | https://doi.org/10.1186/1471-2369-13-160 | |||||||||||||||||||||||
| 関連情報 | ||||||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||||||
| 関連識別子 | 23199000 | |||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||||||||||||
| 収録物識別子 | 1471-2369 | |||||||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||||
| 収録物識別子 | AA12035314 | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Calcium | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Sodium chloride | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Distal tubule | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Na+/Ca2+ exchanger | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Ca2+ channel | |||||||||||||||||||||||
| 主題 | ||||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||||
| 主題 | Claudins | |||||||||||||||||||||||
