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Comprehensive analysis of gene expression of isolated pancreatic islets during pretransplant culture
https://fmu.repo.nii.ac.jp/records/2002015
https://fmu.repo.nii.ac.jp/records/200201504db41cc-d045-4cd7-b89a-87edc6def23d
| 名前 / ファイル | ライセンス | アクション |
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FksmJMedSci_67_p17.pdf (646.9 KB)
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| Item type | デフォルトアイテムタイプ（フル）fmu(1) | |||||||||||||||||||
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| 公開日 | 2021-04-19 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Comprehensive analysis of gene expression of isolated pancreatic islets during pretransplant culture | |||||||||||||||||||
| 言語 | en | |||||||||||||||||||
| 作成者 |
Haga, Junichiro
× Haga, Junichiro
× Sato, Naoya
× Anazawa, Takayuki
× Kimura, Takashi
× Kenjo, Akira
× Gotoh, Mitsukazu
× Marubashi, Shigeru
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| 権利情報 | ||||||||||||||||||||
| 権利情報Resource | https://creativecommons.org/licenses/by-nc-sa/4.0/ | |||||||||||||||||||
| 権利情報 | © 2021 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license. | |||||||||||||||||||
| 内容記述 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | Background: The aim of this study was to investigate the effect of pretransplant culture on the survival of panThe aim of this study was to investigate the effect of pretransplant culture on the survival of pancreatic islet grafts, and to determine the biological characteristics of isolated islets during pretransplant culture. Methods: The survival of islets from Wistar rats, transplanted to diabetic C57BL/B6 mice, was compared between fresh islets and cultured islets. A comprehensive gene expression analysis was employed to investigate biological processes during pretransplant culture, and in vitro validation studies were performed. Results: Survival of cultured xenografts was significantly prolonged as compared to that of fresh islets (fresh:12.5 ± 1.9 days, 1-day cultured:16.0 ± 1.3 days (p= 0.017), 3-day cultured:17.0 ± 2.6 days (p= 0.014)). Comprehensive gene expression analysis identified significant upregulation of annotated functions associated with inflammation in cultured groups. Six proinflammatory genes, including heme oxygenase 1 (HO-1) and IL-6, were significantly upregulated during culture. Validation studies revealed significantly higher levels of IL-6 in the supernatant of cultured islets and HO-1 in the cultured islets when compared with fresh islets. Conclusion: Transplantation of cultured islets induced significant but minimal prolongation of graft survival in xenogeneic combinations. Comprehensive analysis of gene expression in cultured islets showed biological processes associated with proinflammation during culture. | |||||||||||||||||||
| 出版者 | ||||||||||||||||||||
| 出版者 | The Fukushima Society of Medical Science | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| 書誌情報 |
en : Fukushima Journal of Medical Science 巻 67, 号 1, p. 17-26, 発行日 2021 |
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| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | https://doi.org/10.5387/fms.2020-25 | |||||||||||||||||||
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| 識別子タイプ | PMID | |||||||||||||||||||
| 関連識別子 | 33597316 | |||||||||||||||||||
| 関連情報 | ||||||||||||||||||||
| 識別子タイプ | ICHUSHI | |||||||||||||||||||
| 関連識別子 | 2021327588 | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 出版タイプ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||
| 収録物識別子 | 0016-2590 | |||||||||||||||||||
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| 収録物識別子タイプ | EISSN | |||||||||||||||||||
| 収録物識別子 | 2185-4610 | |||||||||||||||||||
| 収録物識別子 | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA0065246X | |||||||||||||||||||
| 主題 | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | islet transplantation | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | heme oxygenase 1 | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | IL-6 | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | culture | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | gene expression | |||||||||||||||||||
