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Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process
https://fmu.repo.nii.ac.jp/records/2001950
https://fmu.repo.nii.ac.jp/records/2001950836d67fd-a1a2-4068-bc16-4c588bf36439
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
FksmJMedSci_64_p15.pdf (6.2 MB)
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| Item type | デフォルトアイテムタイプ（フル）fmu(1) | |||||||||||||
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| 公開日 | 2018-04-23 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process | |||||||||||||
| 言語 | en | |||||||||||||
| 作成者 |
Asai, Emiko
× Asai, Emiko
× Yamamoto, Masaya
× Ueda, Kazuki
× Waguri, Satoshi
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| 権利情報 | ||||||||||||||
| 権利情報 | © 2018 The Fukushima Society of Medical Science | |||||||||||||
| 内容記述 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | To investigate the possible implications of autophagy, one of the degradation pathways induced by metabolic stress, in the dynamic reconstructive process of wound healing, the appearance and changes of punctate structures for microtubule-associated protein 1 light chain 3 (LC3), an autophagosome marker, were examined in a rat skin wound healing model. Although the ratio of LC3-II/LC3-I in Western blotting was not evidently changed during the wound healing process, LC3-positive dots were clearly observed in fibroblasts and myofibroblasts, and occasionally in macrophages, by immunohistofluorescence microscopy. Some of the LC3-positive dots were colocalized with Atg16L signal, an isolation membrane marker, and electron microscopy revealed the presence of typical autophagosomes in fibroblasts near the margin of the wound. The number of LC3-positive dots per fibroblast increased during the later period of the proliferation phase, and interestingly, it was higher in the margin than the center of the wound. It was also high in the periwound skin area. These results suggest that drastic functional changes in fibroblasts during wound healing process are accompanied by the alteration of the autophagy-lysosomal degradation system. | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | The Fukushima Society of Medical Science | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 書誌情報 |
en : Fukushima Journal of Medical Science 巻 64, 号 1, p. 15-22, 発行日 2018 |
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| 関連情報 | ||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.5387/fms.2016-13 | |||||||||||||
| 関連情報 | ||||||||||||||
| 識別子タイプ | PMID | |||||||||||||
| 関連識別子 | 29343655 | |||||||||||||
| 関連情報 | ||||||||||||||
| 識別子タイプ | ICHUSHI | |||||||||||||
| 関連識別子 | 2019024038 | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| 出版タイプ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||
| 収録物識別子 | 0016-2590 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | EISSN | |||||||||||||
| 収録物識別子 | 2185-4610 | |||||||||||||
| 収録物識別子 | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA0065246X | |||||||||||||
| 主題 | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Atg16L | |||||||||||||
| 主題 | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | autophagosome | |||||||||||||
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| 主題Scheme | Other | |||||||||||||
| 主題 | immunohistofluorescence | |||||||||||||
