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  1. 福島医学会
  2. Fukushima Journal of Medical Science
  3. Vol.63 (2017)

Modification of the HCMV-specific IFN-γ release test (QuantiFERON-CMV) and a novel proposal for its application

https://fmu.repo.nii.ac.jp/records/2001932
https://fmu.repo.nii.ac.jp/records/2001932
f9b307e6-6db2-4081-a6dc-c5cf3a7aa214
名前 / ファイル ライセンス アクション
FksmJMedSci_63_p64.pdf FksmJMedSci_63_p64.pdf (2.2 MB)
Item type デフォルトアイテムタイプ(フル)fmu(1)
公開日 2017-08-28
タイトル
タイトル Modification of the HCMV-specific IFN-γ release test (QuantiFERON-CMV) and a novel proposal for its application
言語 en
作成者 Kobayashi, Takahiro

× Kobayashi, Takahiro

en Kobayashi, Takahiro

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Sato, Jun-Ichi

× Sato, Jun-Ichi

en Sato, Jun-Ichi

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Ikuta, Kazufumi

× Ikuta, Kazufumi

en Ikuta, Kazufumi

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Kanno, Ryoko

× Kanno, Ryoko

en Kanno, Ryoko

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Nishiyama, Kyoko

× Nishiyama, Kyoko

en Nishiyama, Kyoko

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Koshizuka, Tetsuo

× Koshizuka, Tetsuo

en Koshizuka, Tetsuo

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Ishioka, Ken

× Ishioka, Ken

en Ishioka, Ken

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Suzutani, Tatsuo

× Suzutani, Tatsuo

en Suzutani, Tatsuo

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権利情報
権利情報 © 2017 The Fukushima Society of Medical Science
内容記述
内容記述タイプ Abstract
内容記述 Human cytomegalovirus (HCMV) is universally distributed among humans without any adverse effects; however, it induces severe diseases in immunocompromised patients such as organ transplant recipients and AIDS patients. To manage these immunocompromised patients, an easy clinical examination for the monitoring of disease risk is required. In this study, we modified the interferon-γ (IFN-γ) release test (QuantiFERON®-CMV) using HCMV immediate early-1 (IE-1) or pp65 whole proteins, or UV-inactivated HCMV particles as an antigen. The response of heparinized peripheral blood from healthy volunteers to the pp65 protein showed an obvious dose-dependent sigmoid curve, although no correlation was observed between results of this assay and an ELISPOT assay. The addition of pp65 to the blood samples at a final concentration of 1×10^3 to 1×10^5 pg/ml was found to be optimum. Using this assay, we observed a significant enhancement in cellular immunity in volunteers after the daily ingestion of yogurt for 8 weeks, which suggested a novel application of the assay in addition to monitoring HCMV infection risk. IFN-γ secretion from peripheral blood cells on HCMV-antigen stimulation differed significantly between individuals; therefore, the assay could not be normalized. Nevertheless, it was found to be particularly useful for observing fluctuations in cellular immune activity on an individual level.
出版者
出版者 The Fukushima Society of Medical Science
言語
言語 eng
書誌情報 en : Fukushima Journal of Medical Science

巻 63, 号 2, p. 64-74, 発行日 2017
関連情報
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 https://doi.org/10.5387/fms.2017-01
関連情報
識別子タイプ PMID
関連識別子 28638004
関連情報
識別子タイプ ICHUSHI
関連識別子 2018243083
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
収録物識別子
収録物識別子タイプ PISSN
収録物識別子 0016-2590
収録物識別子
収録物識別子タイプ EISSN
収録物識別子 2185-4610
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AA0065246X
主題
主題Scheme Other
主題 Human cytomegalovirus
主題
主題Scheme Other
主題 Interferon-γ
主題
主題Scheme Other
主題 Cellular immunity
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