| Item type |
デフォルトアイテムタイプ（フル）fmu(1) |
| 公開日 |
2011-05-26 |
| タイトル |
|
|
タイトル |
Traffic of infused bone marrow cells after genetically-labeled syngeneic bone marrow transplantation following lethal irradiation in mice |
|
言語 |
en |
| 作成者 |
Satoh, Atai
Saito, Takuro
Sato, Yoshihiro
Tsuchiya, Takao
Kenjo, Akira
Kimura, Takashi
Kanno, Ryuzo
Suzuki, Hiroyuki
Kogure, Michio
Hoshino, Yutaka
Gotoh, Mitsukazu
|
| 権利情報 |
|
|
権利情報 |
© 2008 The Fukushima Society of Medical Science |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Bone marrow (BM) cells are considered the source of stem cells for various organs. However, how quickly BM cells can penetrate and constitute lymphoid organs remains elusive. In the present study, we addressed this issue in a model using genetically-labeled syngeneic BM transplantation (BMT). METHODS: Donor BM cells were obtained from "green mice", transgenic mice with enhanced GFP. Lethally irradiated C57BL/6 mice were infused with 1 x 10(6) BM cells from the green mice through the tail vein. BM chimerism was analyzed by FACS and the presence of donor BM cells in thoracoabdominal organs was assessed by fluorescence microscopy. The commitment of BM cells was examined by immunohistochemical staining using epithelium-, macrophage-, B and T-lymphocyte, and endothelium-specific antibodies. RESULTS: BM chimerism reached 40+/- 18.5%, 82.6 +/- 23.4%, and 72 +/- 18% (mean +/- SD) at 1, 4, and 12 wks after BMT, respectively. GFP-positive cells were detected in all organs in the course of chimeric formation. Most GFP-positive cells were T and B lymphocytes in lymphoid systems including spleen, thymus, mesenteric lymph nodes and microvilli, and some were positive for macrophage and endothelial cell markers. CONCLUSIONS: Our results indicate that BM-derived cells migrate rapidly into various thoracoabdominal organs after BMT, and that lymphoid tissues are predominantly replaced with infused BM in lethally-irradiated mice. This confirmed the previous finding by others and suggests high interest of this model for further studies to characterize kinetics and roles of infused cells. |
| 出版者 |
|
|
出版者 |
The Fukushima Society of Medical Science |
| 言語 |
|
|
言語 |
eng |
| 書誌情報 |
en : Fukushima Journal of Medical Science
巻 54,
号 1,
p. 11-24,
発行日 2008-06
|
| 関連情報 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.5387/fms.54.11 |
| 関連情報 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
18924549 |
| 関連情報 |
|
|
|
識別子タイプ |
ICHUSHI |
|
|
関連識別子 |
2009014032 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0016-2590 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2185-4610 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA0065246X |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
chimerism |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
green mouse |
| 主題 |
|
|
主題Scheme |
Other |
|
主題 |
lymphoid organ |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Animals |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Bone Marrow Transplantation |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Cell Movement |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Dose-Response Relationship, Radiation |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Female |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Green Fluorescent Proteins |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Mice |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Mice, Inbred C57BL |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Mice, Transgenic |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Recombinant Proteins |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Transplantation Chimera |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Transplantation, Isogeneic |
| 主題 |
|
|
主題Scheme |
MeSH |
|
主題 |
Whole-Body Irradiation |
FksmJMedSci_54_p11.pdf (2.5 MB)
