{"created":"2024-12-02T08:36:56.470904+00:00","id":2002006,"links":{},"metadata":{"_buckets":{"deposit":"1e80d45e-17d3-434a-b7b7-964a480838d4"},"_deposit":{"created_by":8,"id":"2002006","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2002006"},"status":"published"},"_oai":{"id":"oai:fmu.repo.nii.ac.jp:02002006","sets":["1732778801467:1732778962699:1733128295030"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kariya, Yoshinobu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oyama, Midori","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohtsuka, Mikio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kikuchi, Nobuyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hashimoto, Yasuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yamamoto, Toshiyuki","creatorNameLang":"en"}]}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2020 The Fukushima Society of Medical Science. This article is licensed under a Creative Commons [Attribution-NonCommercial-ShareAlike 4.0 International] license.","subitem_rights_language":"en","subitem_rights_resource":"https://creativecommons.org/licenses/by-nc-sa/4.0/"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"β4 integrin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"glycosylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"squamous cell carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"β1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"6GlcNAc-branched","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"N-glycan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"α6β4 integrin plays pivotal roles in cancer progression in several types of cancers. Our previous study using N-glycan-manipulated cell lines demonstrated that defects in N-glycans or decreased β1,6GlcNAc-branched N-glycans on β4 integrin suppress β4 integrin-mediated cancer cell adhesion, migration, invasion, and tumorigenesis. Furthermore, immunohistochemical analysis has shown that colocalization of β1,6GlcNAc-branched N-glycans with β4 integrin was observed in cutaneous squamous cell carcinoma (SCC) tissue. However, until now there has been no direct evidence that β1,6GlcNAc-branched N-glycans are upregulated on β4 integrin in cutaneous SCC. In the present study, we performed an ELISA analysis of β1,6GlcNAc-branched N-glycans on β4 integrins as well as β4 integrins in cell lysates from human normal skin and cutaneous SCC tissues. The SCC samples showed a 4.9- to 7.4-fold increase in the ratio of β1,6GlcNAc-branched N-glycans to β4 integrin compared with normal skin samples. These findings suggest that the addition of β1,6GlcNAc-branched N-glycans onto β4 integrin was markedly elevated in cutaneous SCC tissue compared to normal skin tissue. The value of β1,6GlcNAc-branched N-glycans on β4 integrin may be useful as a diagnostic marker associated with cutaneous SCC tumor progression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Fukushima Society of Medical Science","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0016-2590","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2185-4610","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"AA0065246X","subitem_source_identifier_type":"NCID"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"123","bibliographicPageStart":"119","bibliographicVolumeNumber":"66","bibliographic_titles":[{"bibliographic_title":"Fukushima Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.5387/fms.2020-12","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"32779579","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2021233987","subitem_relation_type_select":"ICHUSHI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2020-12-11"}],"displaytype":"detail","filename":"FksmJMedSci_66_p119.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://fmu.repo.nii.ac.jp/record/2002006/files/FksmJMedSci_66_p119.pdf"},"version_id":"283c26d4-5347-4dbf-b4be-1ca5496dca08"}]},"item_title":"Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma","item_type_id":"40002","owner":"8","path":["1733128295030"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-12-11"},"publish_date":"2020-12-11","publish_status":"0","recid":"2002006","relation_version_is_last":true,"title":["Quantitative analysis of β1,6GlcNAc-branched N-glycans on β4 integrin in cutaneous squamous cell carcinoma"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-12-02T08:42:38.056737+00:00"}