{"created":"2024-11-29T10:18:09.586370+00:00","id":2001852,"links":{},"metadata":{"_buckets":{"deposit":"9a53d946-3c0a-4b96-aeaa-084d062513b7"},"_deposit":{"created_by":8,"id":"2001852","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2001852"},"status":"published"},"_oai":{"id":"oai:fmu.repo.nii.ac.jp:02001852","sets":["1732778801467:1732778962699:1732874517304"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suganami, Azusa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sakamoto, Kazuho","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ono, Tomoyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Watanabe, Hirotake","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hijioka, Nahoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Murakawa, Masahiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kimura, Junko","creatorNameLang":"en"}]}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2014 The Fukushima Society of Medical Science","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"K+ channel","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Kv2.1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Shakuyakukanzoto","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"H9c2 cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"whole-cell clamp","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Shakuyakukanzoto (shao-yao-gan-cao-tang) is a commonly used Chinese traditional herbal medicine for the treatment of acute pain with muscle cramp. However, its mechanism of action is unclear. We previously reported that a low concentration of Kanzo (licorice) and isoliquiritigenin, a component of licorice, inhibited the potassium (K^+) current in H9c2 cells. Therefore, in the present study, we examined the effects of Shakuyakukanzoto, Shakuyaku or Kanzo on the K^+ current (IKur) in H9c2 cells. Shakuyakukanzoto inhibited IKur in a concentration-dependent manner. The half-maximal concentration of Shakuyakukanzoto was approximately 1.3 mg/mL and the Hill coefficient was 1.2. The order of potency of inhibiting IKur was Kanzo>Shakuyakukanzoto>Shakuyaku. Glycyrrhizin, a major component of licorice, had no inhibitory effect on IKur. A small interfering RNA experiment indicated that IKur was most likely to be Kv2.1 in H9c2 cells. Our results suggest that Shakuyakukanzoto may normalize intracellular and extracellular K^+ balance by inhibiting IKur and reducing K^+ efflux, while the Na^+-K^+ pump promotes K^+ influx into myofibers. Consequently, excess K^+ may be reduced from external space of myofibers. This may be a part of the Shakuyakukanzoto mechanism for improving muscle pain.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Fukushima Society of Medical Science","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0016-2590","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2185-4610","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"AA0065246X","subitem_source_identifier_type":"NCID"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-08-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"30","bibliographicPageStart":"22","bibliographicVolumeNumber":"60","bibliographic_titles":[{"bibliographic_title":"Fukushima Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.5387/fms.2013-16","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"24670676","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2015176968","subitem_relation_type_select":"ICHUSHI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2014-09-19"}],"displaytype":"detail","filename":"FksmJMedSci_60_p22.pdf","filesize":[{"value":"308.8 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://fmu.repo.nii.ac.jp/record/2001852/files/FksmJMedSci_60_p22.pdf"},"version_id":"b4e172ec-4f03-4f0e-bc11-0fa94fdac924"}]},"item_title":"The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells","item_type_id":"40002","owner":"8","path":["1732874517304"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-09-19"},"publish_date":"2014-09-19","publish_status":"0","recid":"2001852","relation_version_is_last":true,"title":["The inhibitory effect of shakuyakukanzoto on K^+ current in H9c2 cells"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-11-29T10:26:50.805596+00:00"}