{"created":"2024-11-29T09:27:30.282176+00:00","id":2001829,"links":{},"metadata":{"_buckets":{"deposit":"cf1949a9-c4c6-4c21-82bd-65484464bdec"},"_deposit":{"created_by":8,"id":"2001829","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2001829"},"status":"published"},"_oai":{"id":"oai:fmu.repo.nii.ac.jp:02001829","sets":["1732778801467:1732778962699:1732871851743"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Inhibitory effect of β-hydroxybutyric acid on L-type Ca^{2+} current under β-adrenergic stimulation in guinea pig Cardiac ventricular myocytes","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kurihara, Masato","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Akama, Youichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kimura, Junko","creatorNameLang":"en"}]}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2012 The Fukushima Society of Medical Science","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"β-hydroxybutyric acid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ketone body","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"L-type Ca2+ current","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cardiac myocyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HCA2 receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Severe ketoacidosis induces heart failure and cardiac arrest, but its mechanism is unknown. Recently, hydroxy-carboxylic acid receptor 2 (HCA(2)) was found to be a receptor for a ketone body, β-hydroxybutyric acid (BHB), and is coupled with Gi-GTP binding protein. HCA(2) expression was reported in the guinea pig heart. Therefore, using guinea pig cardiac myocytes, we investigated effects of BHB on L-type Ca(2+) current pre-augmented with β-adrenoceptor agonist, isoproterenol under the whole-cell voltage clamp. BHB significantly reduced the Ca(2+) current pre-augmented with isoproterenol. The effect of BHB was concentration dependent with IC(50) of 1.1 mM. Nicotinic acid (NA), another ligand for HCA(2), also exerted an effect on the Ca(2+) current similar to that of BHB. The effects of BHB and NA were reduced by a specific Gi inhibitor, pertussis toxin in the pipette solution. Our results suggest that BHB activates Gi-coupled signal transduction pathway via HCA(2) in guinea pig cardiac myocytes. The HCA(2)-mediated signal transduction may be associated with ketoacidosis-induced cardiac suppression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Fukushima Society of Medical Science","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0016-2590","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2185-4610","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"AA0065246X","subitem_source_identifier_type":"NCID"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"150","bibliographicPageStart":"144","bibliographicVolumeNumber":"58","bibliographic_titles":[{"bibliographic_title":"Fukushima Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.5387/fms.58.144","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"23237870","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2013249055","subitem_relation_type_select":"ICHUSHI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2012-12-25"}],"displaytype":"detail","filename":"FksmJMedSci_58_p144.pdf","filesize":[{"value":"391.2 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://fmu.repo.nii.ac.jp/record/2001829/files/FksmJMedSci_58_p144.pdf"},"version_id":"4e3e89e8-317a-4a70-a5de-8bea9bad04dc"}]},"item_title":"Inhibitory effect of β-hydroxybutyric acid on L-type Ca^{2+} current under β-adrenergic stimulation in guinea pig Cardiac ventricular myocytes","item_type_id":"40002","owner":"8","path":["1732871851743"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2012-12-25"},"publish_date":"2012-12-25","publish_status":"0","recid":"2001829","relation_version_is_last":true,"title":["Inhibitory effect of β-hydroxybutyric acid on L-type Ca^{2+} current under β-adrenergic stimulation in guinea pig Cardiac ventricular myocytes"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2025-01-09T09:03:56.506199+00:00"}