{"created":"2024-11-29T05:43:25.626049+00:00","id":2001746,"links":{},"metadata":{"_buckets":{"deposit":"1b0d9dfb-7e7f-42d5-bc90-61565a870e51"},"_deposit":{"created_by":8,"id":"2001746","owners":[8],"pid":{"revision_id":0,"type":"depid","value":"2001746"},"status":"published"},"_oai":{"id":"oai:fmu.repo.nii.ac.jp:02001746","sets":["1732778801467:1732778962699:1732858175774"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Breakthrough of immune self-tolerance to calreticulin induced by CpG-oligodeoxynucleotides as adjuvant","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Abe, Kazumichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ohira, Hiromasa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kobayashi, Hiroko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Saito, Hironobu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takahashi, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Rai, Tsuyoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanno, Yukiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Monoe, Kyoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Watanabe, Hiroshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Irisawa, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sato, Yukio","creatorNameLang":"en"}]}]},"item_1617186499011":{"attribute_name":"Rights","attribute_value_mlt":[{"subitem_rights":"© 2007 The Fukushima Society of Medical Science","subitem_rights_language":"en"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"primary biliary cirrhosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"autoantibody","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hepatocyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Western blot","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Adjuvants, Immunologic","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Amino Acid Sequence","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Blotting, Western","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Calreticulin","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Enzyme-Linked Immunosorbent Assay","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Female","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Hepatitis, Autoimmune","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Immune Tolerance","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Immunization","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Interferon-gamma","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Interleukin-4","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Mice","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Mice, Inbred BALB C","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Mice, Inbred C57BL","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"},{"subitem_subject":"Oligodeoxyribonucleotides","subitem_subject_language":"en","subitem_subject_scheme":"MeSH"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Reportedly, bacterial DNA containing unmethylated cytosine-guanosine dinucleotide motif-containing oligodeoxynucleotides (CpG-ODNs) can induce Th1-type adjuvant effects. We produced autoantibodies and induced hepatitis in mice using extracted proteins from human hepatocytes with CpG-ODNs as adjuvant. Western blot analysis was performed of sera from immunized mice and two patients with autoimmune hepatitis (AIH). When a common band was detected, N-terminal amino acid sequencing was performed to determine its site. For detection of antibodies against the identified protein (calreticulin), ELISA was performed of sera of 50 patients with AIH: 45 with primary biliary cirrhosis (PBC), 24 with chronic hepatitis C (CH), and 24 healthy controls. Mice were immunized with calreticulin protein with CpG-ODNs as adjuvant. Several reacted bands were detected in their sera; in addition, a common band to the sera of patients with AIH was detected at 60 kDa. Subsequent N-terminal amino acid sequencing revealed that the protein was human calreticulin. ELISA showed that, of patients with AIH, PBC, and CH, 30.0% (15/50), 17.8% (8/45), and 12.5% (3/24), respectively, were positive for anti calreticulin antibodies. Splenocytes from immunized mice produced IFN-gamma after they were pulsed with calreticulin protein. Histological analyses of liver specimens taken from mice immunized with calreticulin protein together with CpG-ODNs showed spotty and focal necrosis. Immunofluorescence analysis showed increased expression of calreticulin in the liver treated with CpG-ODNs. These results suggest that a breakthrough of immune self tolerance to calreticulin is induced with CpG-ODNs as adjuvant and that calreticulin protein might be a target antigen in this model.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"The Fukushima Society of Medical Science","subitem_publisher_language":"en"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0016-2590","subitem_source_identifier_type":"PISSN"},{"subitem_source_identifier":"2185-4610","subitem_source_identifier_type":"EISSN"},{"subitem_source_identifier":"AA0065246X","subitem_source_identifier_type":"NCID"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"108","bibliographicPageStart":"95","bibliographicVolumeNumber":"53","bibliographic_titles":[{"bibliographic_title":"Fukushima Journal of Medical Science","bibliographic_titleLang":"en"}]}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617353299429":{"attribute_name":"Relation","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.5387/fms.53.95","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"18402289","subitem_relation_type_select":"PMID"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"2008201401","subitem_relation_type_select":"ICHUSHI"}}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2011-05-26"}],"displaytype":"detail","filename":"FksmJMedSci_53_p95.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://fmu.repo.nii.ac.jp/record/2001746/files/FksmJMedSci_53_p95.pdf"},"version_id":"1d85b69d-5f50-4bb0-b0d1-15b658b643e5"}]},"item_title":"Breakthrough of immune self-tolerance to calreticulin induced by CpG-oligodeoxynucleotides as adjuvant","item_type_id":"40002","owner":"8","path":["1732858175774"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-05-26"},"publish_date":"2011-05-26","publish_status":"0","recid":"2001746","relation_version_is_last":true,"title":["Breakthrough of immune self-tolerance to calreticulin induced by CpG-oligodeoxynucleotides as adjuvant"],"weko_creator_id":"8","weko_shared_id":-1},"updated":"2024-11-29T05:49:37.340906+00:00"}